Precursor-directed biosynthesis of catechol compounds in Acinetobacter bouvetii DSM 14964.

TitlePrecursor-directed biosynthesis of catechol compounds in Acinetobacter bouvetii DSM 14964.
Publication TypeJournal Article
Year of Publication2020
AuthorsReitz ZL, Butler A
JournalChem Commun (Camb)
Volume56
Issue81
Pagination12222-12225
Date Published2020 Oct 13
ISSN1364-548X
KeywordsAcinetobacter, Catechols, Molecular Structure, Stereoisomerism
Abstract

Genome mining for VibH homologs reveals several species of Acinetobacter with a gene cluster that putatively encodes the biosynthesis of catechol siderophores with an amine core. A. bouvetii DSM 14964 produces three novel biscatechol siderophores: propanochelin (1), butanochelin (2), and pentanochelin (3). This strain has a relaxed specificity for the amine substrate, allowing for the biosynthesis of a variety of non-natural siderophore analogs by precursor directed biosynthesis. Of potential synthetic utility, A. bouvetii DSM 14964 condenses 2,3-dihydroxybenzoic acid (2,3-DHB) to allylamine and propargylamine, producing catecholic compounds which bind iron(iii) and may be further modified via thiol-ene or azide-alkyne click chemistry.

DOI10.1039/d0cc04171h
Alternate JournalChem Commun (Camb)
PubMed ID32926028