Imaging Escherichia coli using functionalized core/shell CdSe/CdS quantum dots.

TitleImaging Escherichia coli using functionalized core/shell CdSe/CdS quantum dots.
Publication TypeJournal Article
Year of Publication2006
AuthorsHirschey MD, Han Y-J, Stucky GD, Butler A
JournalJ Biol Inorg Chem
Date Published2006 Jul
KeywordsCadmium Compounds, Escherichia coli, Microscopy, Confocal, Quantum Dots, Selenium Compounds, Staining and Labeling, Sulfides

The internalization of a series of water-soluble CdSe/CdS quantum dots (QDs) stabilized by citrate, isocitrate, succinate, and malate by Escherichia coli is established by epifluorescence and confocal fluorescence scanning microscopy, fluorimetry, and UV-vis spectroscopy on whole and lysed bacterial cells. The organic-acid-stabilized QDs span a range in size from 3.8+/-1.1 to 6.0+/-2.4 nm with emission wavelengths from 540 to 630 nm. QDs of different sizes (i.e., 3.8-6 nm) can enter the bacterium and be detected on different fluorescence channels with little interference from other QDs as a result of the distinct emission profiles (i.e., 540-630 nm, respectively). Costaining QD-labeled E. coli with 4',6-diamidino-2-phenylindole dihydrochloride (DAPI) demonstrates that the QDs and DAPI are colocalized within E. coli, whereas costaining QD-labeled E. coli with membrane dye FM4-64 shows that the FM4-64 is localized in the outer bacterial membrane and that the QDs are inside.

Alternate JournalJ. Biol. Inorg. Chem.
PubMed ID16724226
Grant ListGM38130 / GM / NIGMS NIH HHS / United States