Title | Imaging Escherichia coli using functionalized core/shell CdSe/CdS quantum dots. |
Publication Type | Journal Article |
Year of Publication | 2006 |
Authors | Hirschey MD, Han Y-J, Stucky GD, Butler A |
Journal | J Biol Inorg Chem |
Volume | 11 |
Issue | 5 |
Pagination | 663-9 |
Date Published | 2006 Jul |
ISSN | 0949-8257 |
Keywords | Cadmium Compounds, Escherichia coli, Microscopy, Confocal, Quantum Dots, Selenium Compounds, Staining and Labeling, Sulfides |
Abstract | The internalization of a series of water-soluble CdSe/CdS quantum dots (QDs) stabilized by citrate, isocitrate, succinate, and malate by Escherichia coli is established by epifluorescence and confocal fluorescence scanning microscopy, fluorimetry, and UV-vis spectroscopy on whole and lysed bacterial cells. The organic-acid-stabilized QDs span a range in size from 3.8+/-1.1 to 6.0+/-2.4 nm with emission wavelengths from 540 to 630 nm. QDs of different sizes (i.e., 3.8-6 nm) can enter the bacterium and be detected on different fluorescence channels with little interference from other QDs as a result of the distinct emission profiles (i.e., 540-630 nm, respectively). Costaining QD-labeled E. coli with 4',6-diamidino-2-phenylindole dihydrochloride (DAPI) demonstrates that the QDs and DAPI are colocalized within E. coli, whereas costaining QD-labeled E. coli with membrane dye FM4-64 shows that the FM4-64 is localized in the outer bacterial membrane and that the QDs are inside. |
DOI | 10.1007/s00775-006-0116-7 |
Alternate Journal | J. Biol. Inorg. Chem. |
PubMed ID | 16724226 |
Grant List | GM38130 / GM / NIGMS NIH HHS / United States |